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. 2016 May 12;23:44. doi: 10.1186/s12929-016-0262-3

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© Chuang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Transient induction of p-Drp1(Ser616) by TGI. Hippocampal CA1 samples were collected from the rats at indicated times after 10-min TGI or sham-operated controls followed by protein extraction and western analysis for detection of total Drp1 in (a) and p-Drp1(Ser616) in (b). The same blots were also probed with α-tubulin antibody to serve as an internal reference control for equal loading of proteins in each lane. The ratio change of p-Drp1(Ser616)/total Drp was shown in (c). Values are mean ± SEM from representative blots and quantitative analyses from 4-6 animals in each experimental group are shown. *P < 0.05 versus sham control group in the Scheff′e multiple-range test