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. 2016 May 12;6:25746. doi: 10.1038/srep25746

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Copyright © 2016, Macmillan Publishers Limited

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(a) Western blot analysis of cortical collecting duct lysates. Chronic caffeine intake deceased αENaC expression without significant influence on βENaC, γENaC and sodium chloride cotransporter (NCC) expression (n = 6). (b) Representative curves (left panel) show the individual 24-hour UNaV of rats in control group and caffeine group before and after amiloride injection (3 mg/kg, intraperitoneal). The columns (right panel) show the changes in 24-hour UNaV after amiloride injection (n = 8). (c) Representative curves (left panel) show the individual 24-hour UNaV of rats in control group and caffeine group before and after hydrochlorothiazide (HCTZ) injection (3 mg/kg, intraperitoneal). The columns (right panel) show the changes in 24-hour UNaV after HCTZ injection (n = 8). All data are presented as means ± SEM. *P < 0.05 compared with control.