[Table/Fig-4]:

Immunohistochemistry staining of renal AQP-3. In control group AQP-3 showed focal patchy strong brown cytoplasmic staining in the proximal convoluted tubules (red arrow) and distal convoluted tubules (A) and focal moderate to strong membranous brown staining in the collecting tubules (D). The fructose group revealed higher expression of AQP-3 in the form of diffuse strong brown cytoplasmic and membranous staining in the proximal convoluted tubules, distal convoluted tubules and glomeruli (B) and strong brown membranous (red arrow) and moderate cytoplasmic expression of in the collecting tubules mainly and few of the loop of Henle (E). Fructose plus melatonin group shows patchy moderate brown cytoplasmic staining in the proximal convoluted tubules, distal convoluted tubules and glomeruli (red arrow) (C) and mild to moderate brown membranous expression of AQP-3 in the collecting tubules (F) (A,B,C,D,E and F immunoperoxidase 400XHPF).