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. 2016 May 10;7:11514. doi: 10.1038/ncomms11514

Figure 6. Peri-follicular CD4+ T cells exhibit high migration velocity.

Figure 6

(a) Maximum intensity projection of clustered gDT-II cells (green) around a hair follicle (auto-fluorescent hair in green) at memory (30–70 days) post infection with HSV-1. The dermis is identifiable by collagen (second harmonic generation, blue). Scale bar, 50 μm. (b) Cell migration tracks from a, colours represent confinement ratio, with blue cells being the most confined and red the least. (c) Average confinement ratio and (d) mean velocity of gDT-II cells in the dermal inter-follicular space or peri-follicular clusters at memory following HSV infection. (e) The number of gDT-II cells that enter and exit hair follicle clusters. Symbols in d,e represent individual gDT-II cells. NS, not significant; ****P<0.0001 (two-tailed Mann–Whitney test (ce)). Data is pooled from five movies from five mice (ce). Mean (c,d), mean and s.e.m. (e).