Fig. 7.

Effects of Cav-1 upregulation on the expression and distribution of ZO-1, occludin, and claudin-4 in alveolar epithelial monolayers exposed to hyperoxic conditions. At 60–70 % confluence, alveolar epithelial cells were treated with empty vector and Cav-1 cDNA (1 μg/mL) transfection and then immediately exposed to hyperoxic conditions for 48 h. mRNA (a, b), protein (c, d), and distribution (e) of ZO-1, occludin, and claudin-4 were monitored by RT-PCR, Western blot, and immunofluorescence staining respectively. (e) Green represents ZO-1 and occludin, and red represents claudin-4 (800× magnification). Values are represented as means ± SD, ^## P < 0.01 for comparison with the hyperoxia-treated group, ^** P < 0.01 for comparison with the empty vector-transfected group