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. 2016 May 10;7:11544. doi: 10.1038/ncomms11544

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(a) The LASV GPC is synthesized as a precursor protein. Signal peptidase (SPase) cleaves the small SSP, which remains associated with the complex. SKI protease (SK1/S1P) cleaves GPC into GP1 and GP1. A recombinant LASV (Josiah strain, lineage IV) GPC ectodomain construct (rGPe) was expressed that substituted an uncleavable linker for the SK1 site and deleted the transmembrane and extracellular domains. Constructs expressing recombinant SSP-GP1 (rGP1) and SSP linked to GP2 were also utilized. Molecular weights are indicated in kiloDaltons (kDa). (b) Examples of immunofluorescence assays with unfixed, air-dried cells expressing GP1, GP2 or GPC. All assays were performed two or more times. Scale bar, 10 μm. (c) Distribution of 113 mAbs by subunit specificity, neutralizing activity and reactivity to linear peptides.