Fig. 2.

Example of a video track. The x-axis shows the duration of each trial where a control period is recorded as well as a response period after 5 min. pause in which the mucous extract was added. The y-axis indicates a measure of organ displacements (it can be approximately 1.5 cm for the diverticulum and approximately 1 cm for the copulatory canal). This measure is based on the coordinate output of the marker tracking software, expressed in millions, and shown on the y-axis as values divided by 10⁶. The displacements are counted as contractions and marked with asterisks. In this case the mucus induced one more contraction compared to the control. The maximum intensity reached by the contractions is shown by a dotted line. To count only relevant displacements, a threshold of 25 % difference was fixed between the minimum and maximum point for each trial (dotted line)