Fig. 2.

Lentivirus stably expressed shRNA targeting ZFR in PANC-1 cells. a Evaluation of the lentivirus transduction rate, which was more than 80 % as calculated by cellular enumeration using fluorescence (GFP green fluorescence protein) and light microscopy (Bright). b Quantitative analysis of ZFR knockdown efficiency in PANC-1 pancreatic cancer cells assessed by qPCR (β-actin gene was used as an internal control). c Representative western blot showing ZFR knockdown efficiency determined in PANC-1 pancreatic cancer cells. GAPDH protein was used as an internal control. Data are mean ± S.D. (n = 3; t-test). **p < 0.01; scale bar 100 μm. shCon short hairpin control RNA; sh ZFR short hairpin Zinc finger RNA