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. 2016 May 13;49:26. doi: 10.1186/s40659-016-0086-3

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© Zhao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Depletion of ZFR decreases the viability and proliferation of pancreatic cancer cells. a MTT showing growth curves determined in PANC-1 cells. The number of viable cells was fewer in cells infected with shZFR than in cells infected with shCon. b Representative colony formation showing clonogenic survival determined in PANC-1 cells. c The number of colonies was fewer in cells infected with shZFR than in cells infected with shCon. Data are mean ± S.D. (n = 3; t-test). ***p < 0.001; scale bar 250 μm. shCon short hairpin control RNA; sh ZFR short hairpin Zinc finger RNA