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. 2016 May 12;14:133. doi: 10.1186/s12967-016-0876-y

Fig. 1.

Fig. 1

Graphic representation of applications performed on CTCs cultivated for 14 days. The short-term cultivation provided CTCs available for cytometry evaluation of cell cycle phases distribution, immunofluorescence, protein extraction and cell-sorter protocols. Moreover, conditioned medium (aged 14 days) was collected and cellular production was analyzed