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. 2016 May 12;14:133. doi: 10.1186/s12967-016-0876-y

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© Malara et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Characterization of injected circulating cancer cells and survival trend. Representative dot plots show cell surface CXCR4 expression (x-axis) versus side scatter (SSC; y-axis) of pre-sorting (naive) and post-sort tumour cellular suspensions (a, b). After cell sorting the post-sorting cell population was evaluated for the expression of CK20 reported in histograms (right panels) respectively in localized (a) and advanced (b) colon cancer. c Trends of SDF-1 (data are represented as mean ± standard deviation) in serum of mice injected with eCTCs-CXCR4^posCK^pos segment were reported. In mice injected with eCTCs-CXCR4^posCK^pos derived from localized colon case the levels of serum SDF-1 shows a sharp increase which peaking at days ninety (left panel). In mice injected with eCTCs-CXCR4^posCK^pos derived from advanced colon case the levels of serum SDF-1 show a steeper trend of the curve (right panel)