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. 2016 May 12;14:133. doi: 10.1186/s12967-016-0876-y

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© Malara et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Xenograft developed with circulating tumor cells (eCTCs-CXCR4^posCK20^pos). Immunofluorescence analysis was performed using human monoclonal antibody direct against CK20 antigen. Images of liver derived from mice injected with eCTCs-CXCR4^posCK^pos segment isolated by localized (Loc) and advanced (Adv) colon cases, are reported. Moreover, immunofluorescence analysis for kidney, brain and bone marrow (BM) collected from mice injected with eCTCs-CXCR4^posCK^pos segment derived from localized colon cancer case, is shown. Bright field image, DAPI (blue), CK20 (red) and merge are shown. Scale bars 100 μm