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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: J Hepatol. 2015 Aug 8;63(6):1421–1428. doi: 10.1016/j.jhep.2015.07.034

Fig. 6. Proliferation is significantly reduced following UCHL1 knockdown, inhibition and in UCHL1–/– HSC.

Fig. 6

(A) 3H Thymidine incorporation in (A and B) rat and (C and D) human activated HSC in ± the addition of 20 ng/ml PDGFBB cultured in media (c), DMSO vehicle control (veh) or incremental doses of LDN 57444. (E and F) 3H Thymidine incorporation in 2 separate lines of HSC from UCHL–/– mice or WT littermates ± the addition of 20 ng/ml PDGFBB. 3H thymidine incorporation results are expressed as mean counts/min (cpm) values from triplicate wells (±SEM). Data is representative of 2 (WT and UCHL1–/–) and 3 (human and rat HSC) independent experiments. Multicomparison analysis was performed using two-way repeated measures ANOVA between all groups p <0.05 considered significant; followed by Tukeys post-tests between individual groups where *p <0.05; **p <0.01.