Fig. 3.

Analysis of constitutive of engineered skin structures using a confocal microscope; epidermis is above the white dotted line and dermis is below the line. (A) Cross-sectional H&E stained image of engineered skin. (B) Proliferation of keratinocyte is shown by using proliferation marker, Ki-67 (green). (C) Epidermis-dermis junction is shown by using junctional protein, Laminin-5 (red). (D) Differentiation of keratinocyte is shown by using differentiation markers, involucrin (green) and filaggrin (red). Nucleus of keratinocyte demonstrates using DAPI staining method (blue).