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. 2016 Mar 15;3(2):ofw037. doi: 10.1093/ofid/ofw037

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© The Author 2016. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 4. — Validation of target proteins. Five target proteins were selected for validation via selected reaction monitoring (SRM)-targeted proteomic technique. For each protein, a target peptide was selected, including the following: (A) S100A8 (LLETEC[C]PQYIR), (B) S100A9 (LGHPDTLNQGEFK), (C) GSN (QTQVSVLPEGGETPLFK), and (D) IFI16 (TEPEEVSIEDSAQSDLK). The left panel highlights the peptide intensity-based quantification via label-free analysis for the 5 selected proteins across groups (nonresponder [NR] = 9, responder [R] = 8, and control [C] = 11). Statistical analysis on the normalized data was performed using analysis of variance (ANOVA) model (P ≤ .05) and was visualized using boxplots. The right panel illustrates box-and-whisker plots of the abundance of each peptide via targeted SRM analysis. Statistical analysis on the SRM data was performed using one-way ANOVA, and it is represented by red box for NRs (n = 4), blue box for R (n = 4), and green box for C (n = 8) subjects. *P ≤ .05 and **P ≤ .01 by 2-tailed Student's t test.