Table 2.
Intra-day and Inter-day mean, standard deviation, accuracy and precision for R-bupropion, S-bupropion, 2R,3R-4-hydroxybupropion, 2S,3S-4-hydroxybupropion, erythro-dihydrobupropion A and B, threo-dihydrobupropion A and B.
| R-bupropion and S-bupropion, respectively | ||||||||
| Intra-day | Inter-day | |||||||
|
Theoretical (ng/mL) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
| 0.3 | 0.3, 0.3 | 0.0, 0.0 | 91.6, 91.9 | 12.3, 14.5 | NA | NA | NA | NA |
| 1 | 1.2, 1.2 | 0.1, 0.1 | 80.9, 83.1 | 8.0, 7.4 | 1.0, 1.1 | 0.2, 0.2 | 96.9, 90.0 | 15.7, 16.6 |
| 30 | 33.9, 34.2 | 2.5, 2.8 | 87.1, 86.0 | 7.4, 8.2 | 30.3, 30.1 | 4.7, 4.3 | 98.9, 99.7 | 15.6, 14.4 |
| 200 | 216.2, 215.3 | 10.2, 13.1 | 91.9, 92.3 | 4.7, 6.1 | 211.6, 204.9 | 15.5, 27.9 | 94.2, 97.5 | 7.3, 13.6 |
| 2R,3R-4-hydroxybupropion and 2S,3S-4-hydroxybupropion, respectively | ||||||||
| Intra-day | Inter-day | |||||||
|
Theoretical (ng/mL) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
| 0.3 | 0.3, 0.3 | 0.0, 0.0 | 91.7, 83.6 | 11.5, 4.9 | NA | NA | NA | NA |
| 1 | 1.2, 1.1 | 0.0, 0.1 | 81.0, 86.5 | 4.0, 5.6 | 1.1, 1.0 | 0.2, 0.2 | 93.4, 99.9 | 14.2, 16.6 |
| 30 | 35.6, 35.4 | 1.7, 2.0 | 81.3, 82.0 | 4.8, 5.6 | 29.7, 30.8 | 4.7, 3.9 | 99.0, 97.2 | 15.7, 12.7 |
| 200 | 192.3, 198.5 | 15.4, 13.7 | 96.2, 99.3 | 8.0, 6.9 | 196.0, 205.0 | 23.3, 12.5 | 98.0, 97.5 | 11.9, 6.1 |
| Erythro-dihydrobupropion A and B, respectively | ||||||||
| Intra-day | Inter-day | |||||||
|
Theoretical (ng/mL) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
| 0.15 | 0.14, 0.15 | 0.0, 0.0 | 94.1, 97.8 | 7.6, 14.0 | NA | NA | NA | NA |
| 0.5 | 0.59, 0.58 | 0.0, 0.0 | 81.9, 84.3 | 5.9, 5.6 | 0.5, 0.5 | 0.1, 0.1 | 97.8, 99.4 | 15.0, 11.8 |
| 15 | 17.91, 17.37 | 0.8, 1.1 | 80.6, 84.2 | 4.6, 6.2 | 15.2, 14.5 | 2.0, 2.9 | 98.5, 96.4 | 13.3, 19.9 |
| 100 | 102.43, 111.29 |
13.3, 9.9 | 97.6, 88.7 | 13.0, 8.9 | 95.0, 99.8 | 7.6, 6.1 | 95.0, 99.8 | 8.0, 6.1 |
| Threo-dihydrobupropion A and B, respectively | ||||||||
| Intra-day | Inter-day | |||||||
|
Theoretical (ng/mL) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
Mean (ng/mL) |
s.d. (ng/mL) |
Accuracy (%) |
Precision (% CV) |
| 0.15 | 0.14, 0.18 | 0.0, 0.0 | 90.9, 81.7 | 17.0, 3.7 | NA | NA | NA | NA |
| 0.5 | 0.41, 0.47 | 0.1, 0.1 | 82.3, 93.1 | 12.9, 15.4 | 0.4, 0.5 | 0.1, 0.1 | 88.5, 96.3 | 18.7, 15.6 |
| 15 | 17.72, 17.32 | 0.6, 1.2 | 81.9, 92.3 | 3.4, 6.5 | 14.4, 14.5 | 1.5, 1.9 | 96.0, 96.4 | 10.7, 12.9 |
| 100 | 110.17, 107.72 |
7.5, 7.0 | 89.8, 92.3 | 6.8, 6.5 | 102.7, 105.3 | 9.8, 9.1 | 97.3, 94.7 | 9.6, 8.6 |