Table 1. CD25 antibody-mediated depletion did not significantly affect non-Treg cells.
| Percentage of Live Cells (SD) | |||||
|---|---|---|---|---|---|
| Control mice | Treg depleted mice | ||||
| Cell source | Cell population | Day 7a | Day 14 | Day 7 | Day 14 |
| LRN | B220 (B cells)b | 5.43 (2.4)c | 9.39 (3.4) | 7.84 (2.1) | 11.82 (3.3) |
| CD11c (DC) | 7.50 (4.0) | 9.21 (2.7) | 7.49 (3.9) | 10.19 (2.3) | |
| F4/80 (Mac) | 4.92 (1.0) | 2.81 (1.4) | 3.67 (0.5) | 2.62 (1.5) | |
| DX5 (NK) | 4.88 (2.5) | 4.73 (0.8) | 3.59 (1.5) | 3.96 (0.4) | |
| CD8 (CTL) | 23.97 (22.1) | 24.51 (17.5) | 15.39 (14.2) | 17.37 (11.0) | |
| CD4 (Th) | 46.17 (5.2) | 55.83 (9.1) | 44.82 (6.3) | 49.28 (18.3) | |
| CD4+CD25+FoxP3+ (Treg) | ---- | 4.78 (0.79) | ---- | 1.09 (0.10)* | |
| LUNG | B220 (B cells) | 6.76 (2.6) | 5.69 (1.2) | 6.40 (2.6) | 4.36 (1.0) |
| CD11c (DC) | ----d | 8.66 (0.1) | ---- | 7.43 (0.6) | |
| F4/80 (Mac) | ---- | ---- | ---- | ---- | |
| DX5 (NK) | ---- | ---- | ---- | ---- | |
| CD8 (CTL) | 1.19 (0.4) | 1.52 (0.2) | 1.56 (0.6) | 1.49 (0.4) | |
| CD4 (Th) | 30.83 (7.3) | 42.53 (4.7) | 30.78 (8.9) | 38.34 (4.8) | |
| CD4+CD25+FoxP3+ (Treg) | ---- | 1.65 (0.12) | ---- | 0.91 (0.13)* | |
aDays after infection with M. pulmonis
bAbbreviations: DC- Dendritic Cells; Mac—Macrophages; NK—Natural Killer cells; CTL—Cytotoxic T Lymphocytes; Th—T Helper Cells
cData are expressed as the percentage of live cells as determined by flow cytometry (n = 3).
dData were not collected.
*Significantly different from control mice P ≤ 0.05