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. 2016 May 13;11(5):e0154634. doi: 10.1371/journal.pone.0154634

Fig 3. TALEN activity in N. benthamiana protoplasts.

Fig 3

A) Schematic of ALS2, indicating the TALEN target site (black triangle). The target site is 306 bp downstream of the stop codon. B) Bar graph depicting the frequency (%) of NHEJ-induced mutations created by the ALS2T1 TALEN with the four different UTRs as well as the no-UTR control. Data for both mRNA and DNA constructs are presented. The asterisk denotes a sample that is significantly different from the no-UTR control (p = 0.0065). Error bars denote standard error. C) Representative NHEJ-induced mutations. In the wild-type (WT) sequence, the TALEN binding site is indicated as underlined, bold text. Representative mutations have the number of deleted bases indicated at the right.