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. 2016 May 13;11(5):e0155645. doi: 10.1371/journal.pone.0155645

Fig 9.

Fig 9

Effect of D-Trp(8)-γMSH (0, 50 or 200 nM) on; phospho-NF-κB(p65)Ser276 (A), NF-κB(p65) (B), phospho-Akt (C) Akt (D), IGF-I mRNA (E) and MHC I mRNA (F) in L6 myotubes cell cultures incubated with TNFα (10 μg/ml) or DMEM. Representative Western blots are shown at the middle right. Boxes with immunoblots represent spliced images based on group and treatment order. TNFα increased NF-κB(p65) phosphorylation (P<0.01) and decreased Akt phosphorylation (P<0.05), whereas D-Trp(8)-γMSH prevented those effects. IGF-I mRNA was decreased by TNFα (P<0.05), but not in the cells cultures with TNFα and D-Trp(8)-γMSH. TNFα also decreased MHC I mRNA (P<0.01) and D-Trp(8)-γMSH attenuated this effect. Data are expressed as mean ± SE for n = 6–8 wells per group, *P<0.05, **P<0.01 vs their respective myotube group incubated without TNFα, °P<0.05, °°P<0.01 vs their respective myotube group incubated without D-Trp(8)-γMSH. LSD multiple comparisons test, following one way ANOVA.