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. 2016 May 13;10(5):e0004714. doi: 10.1371/journal.pntd.0004714

Fig 4. Kinetic constants for recombinant Tsf-TbDHFR-TS.

Fig 4

(A) and (B) Determination of DHFR Kmapp values for DHF (4.1 ± 0.6 mM) and NADPH (1.7 ± 0.2 mM), respectively, using purified enzyme. (C) and (D) Determination of TS Kmapp values for dUMP (8.2 ± 0.6 mM) and CH2THF (470 ± 50 mM), respectively, using clarified E. coli lysates. Assays were performed as described in methods. Values represent weighted means of three independent determinations. One representative determination is shown for each Kmapp.