Table 2. Sequences of cAD-like motifs used in MD simulations.
GCN481–100 (original sequence) | MKTVLPIPELDDAVVESFFSSGSGSGS |
cAD-like07 | aceMKTVLPIPELDDAVWESLFSSGSGSGSnme |
cAD-like96 | aceMKTVLPIPELDDAWWWWLFWSGSGSGSnme |
The sequences correspond to the motifs tested experimentally under in vivo conditions [42]. The key hydrophobic residues required for AD function are shown in bold, and the linker sequence used to link the AD to the GCN4 DNA-binding domain in italics. For MD simulations, these structures were created as unfolded polypeptide chains with charge-neutralized termini (ace and nme for N- and C-termini, respectively).