Fig 3. HEXIM knockdown deregulates Hh signaling pathway.

(A) X-Gal staining of hh-lacZ reporter, in WT and rn-Gal4>RNAi Hexim wing discs. For the mutant, β-galactosidase staining duration was reduced to limit signal saturation. En and Hh-LacZ β-galactosidase co-immunodectection in WT (B) and in rn-Gal4>RNAi Hexim (C) wing discs. Arrows indicate the non-autonomous down-regulation of Hh in the posterior compartment of the notum part. The wing pouch is marked with dotted white line. The assays were performed at early L3 stage.