Table 1. Details of the putative defense genes and primer sequences used for RT-PCR.
Accession | Gene name | Protein family | Forward primer (5'-3') | Reverse primer (5'-3') |
---|---|---|---|---|
AJ007348 | PR1.1 | PR1 (basic) | CTGGAGCACGAAGCTGCAG | CGAGTGCTGGAGCTTGCAGT |
Y18212 | PR2 | PR2 | CTCGACATCGGTAACGACCAG | GCGGCGATGTACTTGATGTTC |
AB029934 | Chitinase 1 | PR3 | AGAGATAAGCAAGGCCACGTC | GGTTGCTCACCAGGTCCTTC |
AJ006098 | Wheatwin 1–2 | PR4 | CGAGGATCGTGGACCAGTG | GTCGACGAACTGGTAGTTGACG |
X56011 | TaPERO (Peroxidase) | PR9 | GAGATTCCACAGATGCAAACGAG | GGAGGCCCTTGTTTCTGAATG |
CA684431 | PR10 homolog | PR10 | TTAAACCAGCACGAGAAACATCAG | ATCCTCCCTCGATTATTCTCACG |
CD863039 | PWIR2 | Thaumatin-like | AGGTAATTTTTTTATTGCCCTGTACTG | TTACAGCCGCCGTACTACATGT |
AJ237942 | TaGLP2a | Germin-like | AACAAAGGTGATGTGTTCGTCTTC | GAGCCGGTCTATTGTATTCTTTTCC |
U32428 | WCI2 | lipoxygenase | TAGGAACTGGAACTTCACCGAGC | GGTAGTCCTTGATGTGCAGCGAC |
U32429 | WCI3 | sulfur-rich/thionin-like protein | AAAGTTGGTCTTGCCACTGACTG | TCGACAAAGCACTTCTGGATTTC |
Ta2776 | RNase L inhibitor-like | 68 kDa protein HP68 | CGATTCAGAGCAGCGTATTGTTG | AGTTGGTCGGGTCTCTTCTAAATG |