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. 2016 May 6;11:1959–1971. doi: 10.2147/IJN.S99945

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© 2016 Bai et al. This work is published and licensed by Dove Medical Press Limited

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Overexpression of miR34a by lentivirus vector transfection.

Notes: BEAS-2B cells were transfected by lentivirus vectors with hsa-miR34a or empty lentiviral vectors for 72 hours. As shown in the pictures, the ratio of GFP expression was up to 80%, meaning that transfection efficiency was appropriate (A). We detected the expression of miR34a by real-time PCR, and the result showed that miR34a was successfully upregulated (B). *P<0.05, miR34a compared with miR-NC. Data are expressed as mean ± SD; n=3.

Abbreviations: GFP, green fluorescent protein; miR34a, microRNA 34a; miR-NC, microRNA negative control; SD, standard deviation.