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. 2016 May 13;11(5):e0155726. doi: 10.1371/journal.pone.0155726

Fig 1. Visualization of actin fibers and focal adhesion sites in SMCs overexpressing SMA.

Fig 1

SMCs were transfected with bicistronic lenti virus expressing GFP alone (co, GFP-control), or in addition VSVG-tagged SMA (SMA). (A) Overexpression of SMA was confirmed by Western blot analysis of cell extracts using antibodies against SMA or VSVG. Staining for β-tubulin was included as loading control. Two independent batches of cells were prepared for controls and SMA overexpression. Note the slight up-shift of the SMA band in SMA-SMC-extracts due to the VSVG-tag. (B) GFP-controls or SMA-SMCs were plated onto fibronectin-coated coverslips and stained with phalloidin for F-actin or with antibody for vinculin. A typical micrograph for each condition including a magnification of an area of interest is shown. Bars equal 50 μm.