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. 2016 May 13;11(5):e0155336. doi: 10.1371/journal.pone.0155336

Table 1. Adjusted allele sizes and number allocation for each of eleven VNTR loci and the GP60 marker identified by automated capillary electrophoresis in Cryptosporidium parvum isolates from pre-weaned lambs.

Locus and adjusted fragment size (pb) (allele no.)a No. of isolates (%) (n = 101) No. of farms (n = 46)
ML1 [HGDI = 0.531 (0.449–0.613)] b
226 (1) 65 (64.3) 30
238 (2) 24 (23.8) 15
223 (3) 12 (11.9) 6
TP14 [HGDI = 0.594 (0.530–0.658)]
324 (1) 56 (55.4) 27
333 (2) 26 (25.7) 16
342 (3) 19 (18.8) 13
ML2 [HGDI = 0.702 (0.636–0.768)]
191 (2) 45 (44.5) 24
193 (9) 25 (24.7) 17
195 (15) 2 (1.9) 2
197 (10) 2 (1.9) 1
221 (12) 12 (11.9) 6
225 (16) 5 (4.9) 2
227 (3) 1 (0.9) 1
231 (5) 8 (7.9) 6
237 (8) 1 (0.9) 1
5B12 [HGDI = 0.515 (0.433–0.597)]
167 (2) 27 (26.7) 16
169 (3) 65 (64.3) 33
171 (4) 8 (7.9) 6
165 (1) + 171 1 (0.9) 1
MSB [HGDI = 0.605 (0.551–0.659)]
304 (1) 52 (51.5) 25
310 (5) 33 (32.7) 16
316 (2) 1 (0.9) 1
322 (3) 14 (13.9) 8
304 + 310 1 (0.9) 1
CP47 [HGDI = 0.129 (0.043–0.215)]
417 (1) 94 (93.1) 43
420 (3) 2 (1.9) 1
423 (4) 5 (4.9) 4
MSC6-7 [HGDI = 0.038 (0.000–0.089)]
516 (3) 99 (98) 45
549 (1) 2 (1.9) 1
Cgd3_3850 [HGDI = 0.591 (0.510–0.673)]
157 (7) 24 (23.8) 10
163 (2) 58 (57.4) 29
181 (3) 3 (2.9) 2
193 (4) 10 (9.9) 5
199 (5) 5 (4.9) 4
157 + 193 1 (0.9) 1
Cgd1_3670 [HGDI = 0.444 (0.335–0.552)]
235 (5) 73 (72.3) 33
241 (6) 5 (4.9) 3
247 (7) 1 (0.9) 1
259 (8) 1 (0.9) 1
265 (2) 15 (14.8) 8
277 (9) 6 (5.9) 3
Cgd6_5400 [HGDI = 0.707 (0.643–0.771)]
250 (4) 10 (9.9) 6
262 (5) 5 (4.9) 5
268 (6) 3 (2.9) 3
271 (7) 48 (47.5) 21
277 (1) 7 (6.9) 5
283 (2) 24 (23.8) 14
289 (8) 3 (2.9) 2
277 + 283 1 (0.9) 1
Cgd6_3940 [HGDI = 0.692 (0.621–0.762)]
312 (1) 24 (23.8) 13
324 (4) 4 (3.9) 3
327 (5) 3 (2.9) 2
330 (2) 1 (0.9) 1
333 (6) 8 (7.9) 5
336 (3) 8 (7.9) 5
339 (7) 51 (50.5) 27
342 (8) 2 (1.9) 2
GP60 [HGDI = 0.650 (0.572–0.729)]
339 (7) 54 (53.5) 29
342 (8) 8 (7.9) 5
345 (2) 23 (22.8) 16
348 (9) 3 (2.9) 2
351 (3) 8 (7.9) 7
354 (4) 2 (1.9) 2
360 (10) 1 (0.9) 1
339 + 345 1 (0.9) 1
339 + 351 1 (0.9) 1

a Alleles were compared and numbered consecutively according to those identified within Cryptosporidium isolates from calves and lambs by Quílez et al. [25, 31] and Ramo et al. [32]

b Hunter-Gaston discriminatory power [discriminatory index (95% confidence interval)]