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. Author manuscript; available in PMC: 2017 Apr 20.
Published in final edited form as: Neuron. 2016 Mar 31;90(2):292–307. doi: 10.1016/j.neuron.2016.03.001

Figure 5. Unlike Excitatory Neurons, PV+ cells Do Not Use γCaMKII as a CaM Shuttle.

Figure 5

(A) γCaMKII translocated to the nucleus following 40K for 1 min in excitatory neurons (prevented by nimodipine or CsA), but not PV+ cells.

(B) Images of pCREB staining (red, right panel) in excitatory (top) and PV+ cells (bottom), some transfected with γCaMKII shRNA (green). Transfected excitatory neurons had reduced pCREB compared to control, but transfected PV+ neurons were unaffected. Yellow circles, ROIs used to calculate nuclear pixel intensity. Scale bar, 20 μm.

(C) γCaMKII shRNA knockdown reduced pCREB in excitatory neurons (blue, left y-axis) but not in PV+ cells (red, right y-axis).

(D) 40K did not induce a redistribution of α, β, or δCaMKII within PV+ cells.