Table 2. Intrinsic properties of calb+ and reelin+ cells in juvenile control animals, adult sham control animals, and adult epileptic animals.
See methods section and Fig 3 for descriptions of each of the measured intrinsic properties. Subscript 1 indicates the properties of the first spike in the most depolarized sweep. Properties with a Δ in front of them indicate a difference between the first spike and the subsequent 3 spikes in the most depolarized sweep. Note that because there were no differences in any of the measured parameters between epileptic and age-matched sham control animals, the right-hand column does not display the latter comparison.
| Animal group (and number of animals) | juvenile control (n=13) | adult sham control (n=6) | adult epileptic (n=8) | significant differences | ||||
|---|---|---|---|---|---|---|---|---|
| Properties (see methods for descriptions) | calbindin (n=21) | reelin (n=15) | calbindin (n=5) | reelin (n=11) | calbindin (n=−6) | reelin (n=18) | calb vs reelin | juvenile vs adult |
| RMP (mV) | −62.3±0.5 | −61.4±0.5 | −59.8±1.9 | −60.9±0.6 | −63.3±1.3 | −62.5±1.2 | ||
| Rin (MΩ) | 65.3±3.0 | 52.6±5.3 | 82.3±7.0 | 69.2±6.0 | 80.1±10.8 | 64.9±4.4 | ||
| Saghyperpol (mV) | 2.6±0.1 | 2.9±0.2 | 2.5±0.5 | 3.5±0.3 | 2.7±0.2 | 3.2±0.3 | p<0.0015 | |
| τsag (ms) | 12.1±0.2 | 27.3±0.9 | 45.4±5.3 | 31.3±4.3 | 60.5±7.0 | 40.7±8.1 | p<0.0015 | |
| τmembrane (ms) | 11.0±0.2 | 6.3±0.3 | 12.4±0.5 | 6.8±0.6 | 10.4±2.7 | 8.8±1.0 | p<0.0015 | |
| Rebound amplitude (mV) | 2.3±0.1 | 3.0±0.2 | 1.8±0.4 | 3.4±0.2 | 2.0±0.7 | 3.2±0.3 | p<0.0015 | |
| Sagdepol (mV) | 0.3±0.1 | 3.1±0.6 | 0.4±0.1 | 1.9±0.3 | 0.5±0.1 | 2.1±0.3 | p<0.0015 | |
| Rheobase (pA) | 169±8 | 259±27 | 127±26 | 218±22 | 190±25 | 207±13 | p<0.0015 | |
| Delay to first spike (ms) | 311±42 | 373±79 | 454±93 | 568±88 | 386±134 | 408±69 | ||
| Interspike interval (ms) | 224±12 | 272±27 | 200±13 | 191±27 | 161±15 | 251±19 | ||
| Threshold (mV) | −43.7±0.6 | −42.3±0.8 | −41.0±0.8 | 41.1±1.2 | −40.0±0.9 | −42.3±1.2 | ||
| Spike Amplitude (mV from threshold) | 75.6±1.4 | 70.0±1.5 | 75.5±1.9 | 68.0±2.5 | 67.2±4.1 | 74.3±2.3 | ||
| Spike half-width (ms) | 0.70±0.02 | 0.64±0.02 | 0.63±0.02 | 0.57±0.03 | 0.59±0.04 | 0.70±0.03 | ||
| fAHP amplitude (mV from threshold) | −7.9±0.3 | −8.5±0.4 | −11.0±0.5 | −10.3±0.8 | −12.8±0.6 | −9.8±0.5 | p<0.0015 | |
| sAHP amplitude (mV from threshold) | −10.1±0.3 | −11.3±0.3 | −10.3±0.6 | −11.6±0.7 | −11.0±0.6 | −12.7±0.7 | ||
| DAP amplitude (mV from fAHP) | 0.2±0.1 | 1.2±0.3 | 0.7±0.2 | 2.5±0.6 | 0.9±0.2 | 3.6±0.4 | p<0.0015 | p<0.0015 |
| Threshold1 (mV) | −42.8±0.5 | −40.8±1.0 | −40.5±0.7 | −40.3±1.1 | −37.0±1.6 | −40.8±1.2 | ||
| Amplitude1 (mV) | 72.3±1.2 | 65.1±1.7 | 72.0±2.0 | 64.0±2.4 | 61.9±.4 | 69.3±2.4 | ||
| Halfwidth1 (ms) | 0.67±0.03 | 0.59±0.02 | 0.58±0.02 | 0.55±0.03 | 0.60±0.05 | 0.68±0.04 | ||
| fAHP1 (mV from threshold) | −9.4±0.3 | −8.8±0.5 | −12.3±0.5 | −10.4±0.9 | −14.4±0.9 | −10.5±0.6 | p<0.0015 | p<0.0015 |
| sAHP1 (mV from threshold) | −8.4±0.3 | −3.8±0.9 | −9.2±0.7 | −6.2±0.7 | −9.3±0.5 | −6.9±0.8 | p<0.0015 | |
| DAP1 (mV from fAHP1) | 1.0±0.2 | 5.2±0.7 | −0.9±0.2 | −0.5±0.3 | −0.9±0.3 | −0.2±0.2 | p<0.0015 | p<0.0015 |
| Δ threshold (mV, compared to first spike) | 0.72±0.38 | −0.41±0.38 | −0.70±0.37 | 0.47±0.30 | −1.1±0.5 | 0.1±0.3 | p<0.0015 | |
| Δ spike amplitude (mV, compared to first spike) | 1.2±0.3 | 2.5±0.5 | 1.0±0.6 | 1.1±0.6 | 2.3±0.7 | 1.6±0.4 | ||
| Δ halfwidth (ms, compared to first spike) | 0.10±0.01 | 0.10±0.02 | 0.10±0.01 | 0.07±0.01 | 0.09±0.02 | 0.08±0.02 | ||
| ΔfAHP (mV, compared to first spike) | 2.26±0.19 | 0.03±0.38 | 2.25±0.38 | 0.23±0.44 | 2.6±0.3 | 0.7±0.4 | p<0.0015 | |
| ΔsAHP (mV, compared to first spike) | 0.8±0.2 | −5.3±0.9 | 0.6±0.4 | −3.3±0.7 | 0.4±0.2 | −3.1±0.4 | p<0.0015 | |
| ΔDAP (mV, compared to first spike) | −0.8±0.2 | −4.6±0.7 | −1.6±0.4 | 1.0±0.5 | −2.1±0.4 | 0.5±0.3 | p<0.0015 | |