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. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Nanoscale. 2016 May 3;8(19):10364–10370. doi: 10.1039/c6nr00535g

Fig. 2.

Fig. 2

Confirming the fusion of the βC vesicles onto the nanofibers. (A,B) The βC vesicles were labeled with two distinct fluorescent dyes: DiD (red) in the cell membrane and calcein-AM (green) in the aqueous compartment of the vesicles. (A) Fluorescence emission spectra of the βC vesicles (left panel) and CM-fibers (right panel). (B) Fluorescent images of the CM-fibers in the green channel (left panel) and the red channel (right panel). (C,D) The βC vesicles (labeled with DiD, red) were pre-coated onto PLGA polymeric nanoparticles (labeled with Alexa 488, green) to passivate their fusion ability. (C) Fluorescence emission spectra of the non-fusogenic βC vesicles (left panel) and their mixture with the nanofibers (right panel). (D) Fluorescent images of the nanofibers after incubation with the non-fusogenic βC vesicles in the green channel (left panel) and the red channel (right panel). Scale bars, 25 µm.