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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Cancer Discov. 2012 Feb 28;2(3):260–269. doi: 10.1158/2159-8290.CD-11-0242

Figure 4. nab-paclitaxel and paclitaxel destabilizes cytidine deaminase protein.

Figure 4

A) 40μg of bulk tumor cell lysates were immunoblotted for indicated proteins. B) Immunohistochemistry for cytidine deaminase reveals reduced protein levels in tumour epithelial cells. Scale bar = 50 μm. (n=8) C) RNA isolated from 5 KPC cell lines treated for 36 hours with 10μM paclitaxel was subjected to qRT-PCR, revealing no alterations in mRNA levels compared to controls. RQ values were generated using actin as an endogenous control. D) Protein lysates were generated from the same 5 KPC cell lines treated for 36 hours with DMSO or 10μM paclitaxel and immunoblotted for indicated proteins. Data is representative of four independent experiments. E) Protein lysates were generated from KPC cells pre-treated for 36 hours with DMSO or 10μM paclitaxel followed by 10μM MG132 for 0, 3, 10, or 30 minutes.