Table I.

Primers used for real-time PCR with SYBR-Green.

Gene	Primer sequence	Species	Product size (bp)
ERα	F: 5′-CAT CGA TAA GAA CCG GAG GA-3′	Rat	190
	R: 5′-AAG GTT GGC AGC TCT CAT GT-3′
ERβ	F: 5′-AGC AAC TGG TGC TCA CCC T-3′	Rat	94
	R: 5′-GTC CGC CAG CTT AGT GAG G-3′
ALP	F: 5′-TCC CAC GTT TTC ACG TTT-3′	Rat	140
	R: 5′-GAG ACG TTC TCC CGT TCA C-3′
OCN	F: 5′-TGA GGA CCC TCT CTC TGC TC-3	Rat	150
	R: 5′-AGG TAGCGC CGG AGT CTA TT-3
β-actin	F: 5′-CCC ATC TAT GAG GGT TAC GC-3′	Rat	150
	R: 5′-TTT AAT GTC ACG CAC GAT TTC-3′

F, forward; R, reverse; ER, estrogen receptor; OCN, osteocalcin.