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. Author manuscript; available in PMC: 2016 May 14.
Published in final edited form as: Nat Biotechnol. 2011 Feb 27;29(3):267–272. doi: 10.1038/nbt.1788

Figure 4.

Figure 4

Induction of lung and pharyngeal pouch markers from ventral AFE generated in vitro. (a) Expression of PAX1, NKX2.1, FOXP2, GATA6 and FOXJ1 in HES2-derived cells generated in the two conditions schematically represented on top of the panel (n = 4 to six culture wells from three independent experiments, *, significantly different from WKFBE conditions; P < 0.05). WKFBE: WNT3a, KGF, FGF10, BMP4 and EGF. (b) Induction of SFTPC and GCM2 mRNA in ventralized AFE in the presence of factors indicated in the figure.