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Illustration of prolonged suppression of rhythmic inspiratory activity by bilateral microinjection of muscimol (10 µm) in the pre-BötC (yellow shaded area) of juvenile rat perfused brainstem–spinal cord in situ. As in vivo, muscimol rapidly reduces phrenic nerve inspiratory discharge frequency and terminates inspiratory activity. ∫PN, phrenic nerve integrated activity; fR, respiratory frequency.
