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. 2016 Mar 2;57(5):1058–1068. doi: 10.1093/pcp/pcw049

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© The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Target sequences on OsDMC1 genes. (a) Target sites on OsDMC1A. Blue arrowhead, gRNA recognizes the target site on OsDMC1A and OsDMC1B; red arrowhead, gRNA recognizes the target site on OsDMC1A; cyan bar, second exon of OsDMC1A. (b) Naming rules for gRNAs on OsDMC1A. (c) Homology and mismatch of target sequences in OsDMC1A and OsDMC1B. Mismatches to the target sequence on OsDMC1 are shown in red. The green arrowhead indicates the expected cleavage site. Restriction enzyme recognition sequences are underlined.