Table 2.

Mutation variations of pZH_p-gRNA_MMCas9-Nuclease transformed calli in the DMC1A gene

Paired gRNAs		AB4t+AB3b	A2t+AB3b	A1t+AB3b	AB4t+A6b
Distance of paired gRNAs		37bp	71bp	99bp	66bp
	Recognition strand
Total number of mutated clones	Top-strand mutation	2	3	0	12
		(4.5%)	(6.2%)	(0%)	(25%)
	Bottom-strand mutation	6	3	25	1
		(13.6%)	(6.2%)	(52%)	(2%)
	Top- and Bottom-strand mutations	7	15	1	7
		(15.9%)	(31.2%)	(2.2%)	(14.5%)
	Deletion between two target sites	22	20	0	9
		(50%)	(41.6%)	(0%)	(18.7%)
Number of no-mutated clones		7	7	22	17
		(15.9%)	(14.5%)	(45.8%)	(35.4%)
Total number of sequenced clones		44	48	48	48