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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Stem Cells. 2015 May 13;33(8):2574–2585. doi: 10.1002/stem.2022

Figure 6.

Figure 6

CXCR7 interacts with Rac1 to induce neural progenitor cell (NPC) migration. (A): Cxcr4−/− NPCs were seeded on bovine serum albumin (BSA)/CXCL12 stripes and cell lysates were collected at the indicated time points and subjected to Western blotting for total Rac1 and Rac1-GTP detection. Actin was used as loading control. (B): Fold changes in Rac1GTP were normalized as a ratio of Rac1GTP to total Rac1 after densimetrical quantification and shown as fold changes relative to 0 minute time point. (C): Cxcr4−/− NPCs were treated with CXCL12 at the indicated time points. Cell lysates were treated with anti-CXCR7 antibody to immunoprecipitate CXCR7-Rac1 and CXCR7-β-arrestin2. Protein levels of β-arrestin2 and Rac1 were determined through immunoblotting. (D): NPCs on transwell insert were incubated under the indicated conditions in the presence of CXCL12 or NSC23766. Migrated cell numbers of each condition were normalized to the negative control and quantified as migration index. ***, p <0.001. Abbreviation: IP, immunoprecipitation.