Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2017 May 1.

Published in final edited form as: DNA Repair (Amst). 2016 Mar 29;41:32–41. doi: 10.1016/j.dnarep.2016.03.009

Nicorandil attenuates cisplatin-induced phosphor-H2AX and partially blocks cell death but does not alter cisplatin-induced changes in CGRP release. (A) The top panels show representative Western blots of phospho-H2AX (p-H2AX) and vinculin from cultures prior to and after 24 and 48 hours of exposure to 10 μM cisplatin after various drug treatments as indicated. The bottom panels represent the densitometry of p-H2AX expression normalized to vinculin from 3 independent experiments. The columns represent the mean ± SEM from cultures treated as indicated. An asterisk indicates a statistically significant increase in p-H2AX density compared to medium-treated controls, whereas a cross indicates a statistically significant reduction in p-H2AX compared to medium-treated controls. (B) Each column represents the mean ± SEM of percent survival of cells from control cultures (open columns) and those treated with various concentrations of cisplatin for 24 hours. Each panel represents cultures pretreated with medium, nicorandil, daidzin, or nicorandil and daidzin as indicated. An asterisk indicates a significant difference in survival after cisplatin compared to controls, whereas a cross indicates a significant difference in cultures treated with daidzin and nicorandil using ANOVA and Tukey's post hoc test. (C) Each column represents the mean ± SEM of basal CGRP release (open columns) or capsaicin-stimulated release (shaded columns) in fmol/well/min for untreated sensory neurons in culture (Medium) or cultures treated with daidzin or daidzin and nicorandil as indicated. Cultures were treated with medium (controls) or 10 μM cisplatin for 24 hours prior to release experiments. For release, wells of cells from 3 independent harvests were exposed for 10 min to HEPES alone (basal; open columns), or HEPES in the presence of 30 nM capsaicin (solid columns) as indicated. An asterisk indicates a significant difference in capsaicin-stimulated release compared to untreated cells.

Nicorandil attenuates cisplatin-induced phosphor-H2AX and partially blocks cell death but does not alter cisplatin-induced changes in CGRP release. (A) The top panels show representative Western blots of phospho-H2AX (p-H2AX) and vinculin from cultures prior to and after 24 and 48 hours of exposure to 10 μM cisplatin after various drug treatments as indicated. The bottom panels represent the densitometry of p-H2AX expression normalized to vinculin from 3 independent experiments. The columns represent the mean ± SEM from cultures treated as indicated. An asterisk indicates a statistically significant increase in p-H2AX density compared to medium-treated controls, whereas a cross indicates a statistically significant reduction in p-H2AX compared to medium-treated controls. (B) Each column represents the mean ± SEM of percent survival of cells from control cultures (open columns) and those treated with various concentrations of cisplatin for 24 hours. Each panel represents cultures pretreated with medium, nicorandil, daidzin, or nicorandil and daidzin as indicated. An asterisk indicates a significant difference in survival after cisplatin compared to controls, whereas a cross indicates a significant difference in cultures treated with daidzin and nicorandil using ANOVA and Tukey's post hoc test. (C) Each column represents the mean ± SEM of basal CGRP release (open columns) or capsaicin-stimulated release (shaded columns) in fmol/well/min for untreated sensory neurons in culture (Medium) or cultures treated with daidzin or daidzin and nicorandil as indicated. Cultures were treated with medium (controls) or 10 μM cisplatin for 24 hours prior to release experiments. For release, wells of cells from 3 independent harvests were exposed for 10 min to HEPES alone (basal; open columns), or HEPES in the presence of 30 nM capsaicin (solid columns) as indicated. An asterisk indicates a significant difference in capsaicin-stimulated release compared to untreated cells.