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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 29;41:32–41. doi: 10.1016/j.dnarep.2016.03.009

Table 1. Effect of nicornadil on enzyme kinetic parameters.

Enzyme h kcat * × 10-6 (s-1) KM (nM) kcat/KM (s-1) Fold-increase#
Wild-type 1.0 0.77 +/- 0.038 15 +/- 3 5.1 × 104
Wild-type + nicorandil 1.7 1.1 +/- 0.046 13 +/- 1 9 × 104 1.7
R177A 1.0 5.4 +/- 0.034 28 +/- 6 19 × 104 3.8
R177A + nicorandil 1.2 9.1 +/- 0.40 14 +/- 2 67 × 104 3.4

h refers to the Hill slope calculated for an allosteric sigmoidal kinetics model in GraphPad.

*

kcat values are divided by 106 and were calculated as Vmax/[enzyme (nM)]. Vmax is in relative fluorescence units per second. Active enzyme concentration was estimated as the total enzyme concentration in this case.

# Fold increase is reported for wild-type stimulated by nicorandil vs. wild-type alone, R177A vs. wild-type, and R177A stimulated by nicroandil vs. R177A APE1 alone.