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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2016 Mar 30;95:308–322. doi: 10.1016/j.freeradbiomed.2016.03.031

Figure 3. Inhibition of antioxidant enzymes sensitizes cancer cell lines and primary hepatocytes to ascorbate mediated cytotoxicity.

Figure 3

Hep G2 cells (A), T-47D cells (B), MIA PaCa-2 cells (C), and primary hepatocytes (D) were preincubated for one hour with no inhibitors, the catalase inhibitor 3-amino-1,2,4-triazole (AT) (5 mM), the glutathione reductase/thioredoxin reductase inhibitor BCNU/carmustine (100 μM), or both inhibitors, followed by a two hour treatment with different concentrations of ascorbate (1, 3, 5, 10, and 20 mM). Cell viability was measured through CellTiter-Glo cell viability assay. Error bars represent SEM (n=3). Activity levels of catalase (E) and Glutathione peroxidase (F) in rat liver tissue, primary rat hepatocytes, Hep G2, Huh-7, SNU-449, T-47D, and MIA PaCa-2 cells. Error bars represent SEM (n=4). Some error bars may be smaller than the symbol.