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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mol Cancer Res. 2016 Mar 15;14(5):437–447. doi: 10.1158/1541-7786.MCR-15-0348

Figure 4.

Figure 4

Inhibition of stress-associated MAPK signaling attenuates SASP expression in CAFs. A, N-CAFs were treated with 100nM GEM plus DMSO or the above listed small molecule inhibitors for 48h and SASP mediator expression was compared to untreated cells. Drug concentrations and targets: SB203580 10μM- P38 MAPK, SP600125 20μM- JNK, BEZ235 1μM- ATM/ATR/DNA-PK/PI3K/mTOR, AZD77622 200nM- Chk1/Chk2 VE821 2μM- ATR, KU55933 20μM- ATM. B, cell lysates from N-CAFs treated with 100nM GEM and DMSO, SB203580 or SP600125 at the indicated concentrations (μM) were blotted for P38 MAPK and JNK activation as well as their downstream targets, HSP27 and cJUN, respectively. Lysates from untreated N-CAFs were used as a control. Densitometry values are displayed below the blots. All values were normalized to GAPDH, and expression is displayed as the ratio of phospho- to total protein. For all experiments, data represent the composite of two separately performed experiments. The blots displayed in B are from one representative experiment.