Figure 3. δT-13’-COOH (δT-13’) profoundly modulated sphingolipid metabolism in HCT-116 cells in time- and dose-dependent manners.
HCT-116 cells were treated with 10 or 20 μM of δT-13’-COOH for 1, 2, 4, 8, or 16 h. LC-MS/MS was used to measure the sphingolipid levels including total dhCers (A), C16:0-dhCer (B), dhSph (C), total Cers, C16:0-Cer, C18:0-Cer (D) and total SMs (E). Results are shown as mean SEM for at least three independent experiments. *p < 0.05 and **p < 0.01 indicate a significant difference between treated and DMSO control cells at each indicated time.