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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Cancer Res. 2016 Feb 26;14(5):493–503. doi: 10.1158/1541-7786.MCR-15-0498

Figure 3. Src-kinase is associated with CXCR7-mediated EGFR transactivation.

Figure 3

A : Growth factor starved RWPE-1 and RWCX7 cells were incubated with the Src inhibitor PP2 or vehicle control (ethanol) followed by stimulation with EGF (10 ng/ml, 5 min). Cell lysates were subjected to Western blot analysis using anti-pEGFR and anti-ERK1/2 antibodies. B: β-AR2 was overexpressed or CXCR7 was downregulated in LNCaP cells. Then, cells were incubated with the Src inhibitor PP2 or vehicle control (ethanol) and subjected to western blot analysis following stimulation with EGF (10 ng/ml, 5 min). OE= Over expression. C: LNCaP cells with stable β-AR2 down regulation (1, 2, and 3) and control (13, Control-shRNA) were subjected to western blot analysis for evaluation of Src phosphorylation/activation. In parallel experiments, CXCR7 was downregulated with siRNA or overexpressed (right panel).