Figure 3. PKR activation with C134 and association of productivity with STAT1 response.
PKR and eIF2α in human cell lines (A) was assessed by western blot for phosphorylation in response to mock or C134 (MOI=1, 12 hpi) infection. Cell lines were assessed in a multistep infection assay (MOI=0.1, 48 hpi) with C134 and M2001 for the percentage of cells positive for GFP (B and D) and the infected cell count as a percentage of the mock infected cell count (C and E). Data were collected in triplicate and the mean reported. Cell lines were sorted based on their STAT1 response and tested for statistical significance using the Mann-Whitney U test with the medians and interquartile range plotted.