Skip to main content
. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Cancer Res. 2016 Feb 16;14(5):482–492. doi: 10.1158/1541-7786.MCR-15-0427

Figure 5. Basal expression of ISGs in MPNST cell lines and effect of increased ISG expression on viral productivity.

Figure 5

Basal expression of ISGs in all human MPSNT cell lines was assessed by western blot (A). S462-luc cells were treated with IFN-β for 24 hr and subsequently probed by immunoblot for ISGs (B) or infected with C101 at MOI=0.2. Infected cells were then assessed by FACS for the percentage of cells positive for viral eGFP at 48 hpi (B). S462-luc was stably transduced with a control lentivirus (DsRed2) or lentiviruses encoding the transcription factors STAT1-FLAG, STAT2 and IRF9 which compose the ISGF3 complex were assessed for ISG expression by western blot (C). Multi-step (MOI=0.1, 48 hpi) and single-step (MOI=1, 24 hpi) (D) viral recovery assays with R3616 were conducted in control and ISGF3 transduced cell lines. In each described assay, data were collected in triplicate and the standard deviation reported. The log-fold change is reported under the significance indicator.