Fig. 6.

Comparable epithelial Na⁺ channel (ENaC) activity and aldosterone in low fat- and high fat-fed mice. A: net transepithelial Na⁺ transport (J_Na) under low or high flow conditions of isolated cortical collecting duct segments from mice on a NSD. Positive deflection indicates Na⁺ absorption. n = 6 tubules/group. B: 24-h urinary aldosterone excretion on a NSD or HSD. C and D: urinary Na⁺ (U_Na; C) and urinary K⁺ (U_K; D) excretion. E: ratio of U_Na to U_K in mice after 4 h of vehicle and intraperitoneal benzamil on a NSD or HSD. N = 8 mice/group. *P < 0.05 compared with LFD-fed mice; #P < 0.05 compared with low flow (A) or vehicle (C–E).