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. 2016 Feb 25;310(9):G716–G725. doi: 10.1152/ajpgi.00273.2015

Fig. 6.

Fig. 6.

The number of endothelial cells is decreased in NEC intestine tissues and further decreased when the VEGFR2 pathway is inhibited. Intestinal tissue cells were dissociated with EDTA followed by collagenase digestion. Single-cell suspensions were generated, stained with indicated markers, and analyzed by flow cytometry. The percentage of CD31+ cells in the live, and nonepithelial- and nonleukocyte-derived cell populations is shown. A: representative histograms showing decreased percentage of CD31+ cells in the NEC (middle) and NEC/SU5416 (right) groups compared with DF controls (left). B: bar graph showing average values of multiple samples including A (n = 3 in each group; *P < 0.05, **P < 0.005). Results were similar in 2 separate experiments. C: representative histogram showing a decrease in percentage of CD31+ cells in DF pups injected with SU5416 (right) compared with DF controls (left). D: bar graph showing average values of multiple samples including C (n = 6 in each group; **P < 0.01). Results were similar in 2 separate experiments.