Fig. 11.

NAC feeding blocks γ-IR-induced protein thiol oxidation in mouse intestine. Adult mice were fed liquid diet with or without 20 mM NAC for 5 days before TBI (IR) or sham treatment (Sham). At 2 h post-IR, the levels of reduced and oxidized protein thiols in distal colon (A and B) and ileum (C and D) were measured as described in materials and methods. Fluorescence images were collected by confocal microscopy (A and C). Fluorescence density was evaluated by using Image J software (B and D). Values are means ± SE (n = 5). *Significantly (P < 0.05) different from corresponding value for Sham-treated mice. #Significantly different from corresponding value for the IR group.