Fig. 4.

Sirt3-deficiency promotes Doxo-induced cardiac injury. A: representative heart sections stained with Masson's trichrome for determining fibrosis in WT and Sirt3.KO mice treated with Doxo. B: quantification of cardiac fibrosis in different group of mice treated or not treated with Doxo. C: collagen and ANF mRNA levels in the hearts of WT and Sirt3.KO mice treated with vehicle or Doxo. Values are means ± SE; n = 5. D: representative electron microscopy images of the heart from WT and Sirt3.KO mice treated with Doxo or vehicle. Scale bar = 2 μm. E: total heart lysate from different group of mice was analyzed by Western blotting using pan acetyl lysine (Ac.K) and MnSOD antibody.