Fig. 5.

SIRT3 overexpression mitigates Doxo-induced mitochondrial (mt)DNA damage. A: primary cultures of cardiac fibroblasts obtained from WT and Sirt3.KO mice were treated with Doxo for 24 h. mtDNA damage was assessed by quantitative PCR analysis. *Significantly different (P < 0.01) from fibroblasts of WT mice. B: primary cultures of rat neonatal cardiomyocytes were infected with adenovirus expressing SIRT3 (Ad.SIRT3) or empty adenovirus (Ad.mk). Following 24 h of infection, cells were treated with different doses of Doxo as indicated and mtDNA damage was assessed. *Significantly different (P < 0.01) from cells infected with empty virus. C: mitochondrial DNA damage was assessed in whole heart of WT and Sirt3.KO mice treated with saline or Doxo. D: mtDNA damage was assessed in whole heart of control (ntg) and Sirt3.tg mice treated with saline or Doxo. All values are means ± SE; n = 5–7.