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. 2016 Feb 12;310(8):H962–H972. doi: 10.1152/ajpheart.00832.2015

Fig. 6.

Fig. 6.

Sirt3 deacetylates and prevents Doxo-mediated degradation of oxoguanine-DNA glycosylase-1 (OGG1) in the mouse heart. A: cell lysate of cardiomyocytes overexpressed with SIRT3 was subjected to immunoprecipitation (IP) with indicated antibodies. The resulting beads were analyzed by immunoblotting with anti-OGG1 or anti-SIRT3 antibodies. Note; SIRT3 binds to OGG1 in cardiomyocytes. B: primary cultures of rat cardiomyocytes were treated with 1 uM Doxo for 24 h. Cells were harvested and cell lysate was subjected to immunoprecipitation with anti-OGG1 antibody. The resulting beads were analyzed by immunoblotting with anti-acetyl lysine antibody. C: quantification of relative acetylated OGG1 levels in vehicle and Doxo-treated cardiomyocytes. D: heart lysates of Sirt3.tg and control (ntg) mice were subjected to immunoprecipitation with anti-OGG1 antibody. The resulting beads were analyzed by immunoblotting with anti-acetyl lysine antibody. Note: Sirt3 deacetylates OGG1 in the heart. E: heart lysate of WT and Sirt3.KO mice treated with vehicle or Doxo were subjected to immunoblotting with indicated antibodies. G: heart lysate of control, ntg, and Sirt3.tg mice treated with vehicle or Doxo were subjected to immunoblotting with indicated antibodies. Results are shown for 2 animals in each group. F and H: quantification of OGG1 levels in different group of hearts. Values are means ± SE; n = 5.